不同水温下儿茶素EGCG在黄颡鱼(Pelteobagrus vachelli)体内代谢动力学和肝消除研究     

葛建  刘洋  李明揆  赵进  韩宝瑜 《海洋与湖沼》2012,43(5):938-942

采用反相高效液相色谱-紫外检测法测定了黄颡鱼血浆、肝脏中儿茶素EGCG含量,并研究了不同水温(10℃和20℃)下EGCG在黄颡鱼体内代谢动力学和肝组织消除特征。结果表明,高温下黄颡鱼腹腔注射EGCG后体内吸收较快,T1/2ka为0.21h,T1/2α为2.07h,T1/2β为22.89h,AUC为5195.41μg·h/ml,Tp为0.95h,Cmax为257.75μg/ml;低温下分别为5.67h、9.53h、207.49h、17283.03μg·h/ml、12.56h和117.16μg/ml。同时在0.5、1、2、4、8、12、24、48、96h各时间点检测了肝脏组织中EGCG浓度,并计算得出高温下EGCG在肝组织中的T1/2β为24.15h,低温下肝组织中T1/2β为64.17h。对不同水温下代谢动力学和肝组织消除规律的研究表明,EGCG在黄颡鱼体内吸收、分布和消除速度与水温关系密切,高温下均较低温下迅速。  相似文献   

Study on the high precision acoustic measurement techniques for determining temperature field around seafloor hydrothermal vent     

蔡勇  樊炜  周艳  付现桥  方辉  金涛 《中国海洋工程》2012,26(4):723-732

This paper presents the basis of acoustic method used for temperature field measurement of seafloor hydrothermal vent and two techniques of the parabolic interpolation and the bending compensation of propagation paths of acoustic signal are introduced. Experimental research is performed to exactly rebuild the temperature field around hot springs on the floor of Qiezishan Lake, Yunnan, China. The accuracy of the travel time estimation has been improved based on the aforementioned technique and method. At the same time, by comparison of the results of temperature field with different means, the max absolute error, the maximum relative error and the root mean square error are given. It shows that the technique and the method presented in the paper can be applied to the temperature field measurement detector around the seafloor hydrothermal vent. It also has a good accuracy.  相似文献   

象山港养殖大黄鱼寄生新贝尼登虫成虫形态学和28S rDNA,ITS1分子鉴定     

苗亮  李明云  蒋进  丁文超  陈炯  史雨红 《海洋学报》2012,34(2):122-128

贝尼登类单殖吸虫是象山港海水养殖鱼类中一类危害严重的寄生虫。应用PCR扩增及DNA序列分析的分子生物学手段,并结合对成虫的形态学分析,对象山港养殖大黄鱼体表寄生的贝尼登类单殖吸虫(记作XSp)进行了种类鉴定,结果表明XSp从形态特征上属于新贝尼登虫属,与梅氏新贝尼登虫高度相似。扩增得到XSp的28S rDNA和ITS1序列长度分别为393和427 bp,与梅氏新贝尼登虫和鱾新贝尼登虫的5个28S rDNA 序列、2个ITS1序列的比对分析显示相似性除1个为97.4%外其余均大于99%,提示XSp与这几个鱾新贝尼登虫和梅氏新贝尼登虫为种内关系,而XSp与贝尼登虫的3个28S rDNA 和1个ITS1序列相似性分别为84.3%~89.5%和60.2%。系统进化树显示该吸虫与梅氏新贝尼登虫和鱾新贝尼登虫形成一个紧密的簇,而与贝尼登虫亲缘关系较远。根据普通生物学和序列特征分析,将XSp定种为梅氏新贝尼登虫,并且支持Whittington和Horton (1996)提出的梅氏新贝尼登虫和鱾新贝尼登虫为同种异名的分类观点。  相似文献   

浙江沿海2010“10.25”温带风暴潮灾害特征及成因分析     

丁骏  王晶  王勤 《海洋预报》2012,29(4):23-28

2010年10月25—29日,浙江省沿岸遭受了本海区近10年来最强温带风暴潮袭击,浙江北部沿海部分岸段出现超过当地警戒潮位的高潮位,宁波镇海、舟山定海和沈家门部分沿岸地区出现漫滩和内涝等险情。本文根据实况天气图、实况观测资料和实况分析资料,分析了10.25温带风暴潮的成因、特点,为今后预报此类风暴潮提供一些可借鉴的预报经验。  相似文献   

一次“双谷”型ENSO冷事件的成功预测     

李进  丁婷  单晓龙  钱维宏 《海洋预报》2012,29(6):1-7

2011年春季利用基于Morlet小波分析的余弦函数叠加法对中东太平洋的ENSO冷事件(La Nina)未来发展进行预测,结果成功地预测出了一次呈现"双谷"型的ENSO冷事件发展过程。此方法拟合的1980年1月—2011年2月的Nino3.4指数序列与原序列的相关系数为0.68;对2000—2011年间7次ENSO事件预测结果检验,发现提前3、6、9和12个月时Nino3.4指数的平均预报相关系数(R)分别为0.79,0.81,0.83和0.64,平均预报技巧得分(SS)分别为0.87,0.80,0.80和0.27。此方法具有计算简便,易于运行,预报时效稳定的特点,特别在提前6—9个月时对ENSO的预测仍有较好效果。  相似文献   

10kW漂浮点吸收直线发电波力装置   总被引：1，自引：0，他引：1  

吴必军  刁向红  王坤林  游亚戈  张运秋  孙洲平  肖阳  胡国葆  贾海媛  谌瑾 《海洋技术学报》2012,(3):68-73

点吸收式是目前世界上发展较多、转换效率较高的一种波浪能利用技术,介绍我国自主研发的一台10 kW漂浮点吸收波浪能直线发电装置。该装置主要由4 m直径蝶形振荡浮子、6 m直径水下阻尼板、1 t重直线发电机组成,整个装置漂浮在海面上,是一个钢结构体。装置外形为煤油灯形,采用共振聚波和阻尼匹配技术提高装置的转换效率,有效发电时间不受来波方向和潮位的影响,针对构成装置的不同材料采用不同的防腐技术提高装置的整体抗海水腐蚀能力,采用下潜方式躲避台风打击,采用蓄能技术提高锚泊系统抗走锚和断链能力。直线发电机是一个圆筒型结构体,具有较高的转换效率和防海水腐蚀能力。发电装置进行了实海况试验,为后续的研究工作提供了相关经验。  相似文献   

胶州湾大沽河口潮间带沉积物中叶绿素和有机质含量的季节变化   总被引：1，自引：0，他引：1  

尹盛乐  刘晓收  袁超  谭朋  胡金  刘阳  董树刚 《海洋湖沼通报》2012,(2):97-106

为了解胶州湾大沽河口潮间带沉积物中底栖微藻和有机质含量的季节变化,于2010年4月至2011年2月,分4次(春、夏、秋、冬)测定分析了沉积物中的叶绿素含量和有机质含量,并分析了沉积物的粒度组成及现场温度。结果表明,表层(0～2cm)沉积物叶绿素a含量为0.42～6.06mg/kg,有机质含量为0.48%～3.71%;次表层(2～5cm)叶绿素a含量为0.13～3.53mg/kg,有机碳质量为0.31%～3.78%;深层(5～8cm)叶绿素a含量为0.14～1.95mg/kg,有机质含量为0.47%～3.72%。经统计检验和Pearson相关性分析得出,胶州湾大沽河口沉积物中的叶绿素a含量春冬两季最高,夏季和秋季最低;叶绿素a含量与深度和温度呈负相关;与所处潮带、有机质含量、粉砂黏土含量在本实验中均无显著的相关性;有机质含量与季节关系不大,高潮带最高,其含量与粉砂黏土含量呈正相关,与叶绿素含量、深度和温度关系不明显。  相似文献   

鉴定海参的斑点杂交法及其应用     

律迎春  段高飞  左涛  徐洁  李晋  郑荣  唐庆娟  薛长湖 《中国海洋大学学报(自然科学版)》2012,(11):62-65

本研究目的是通过斑点杂交方法对不同种类的海参进行快速准确的鉴定。采用CTAB沉淀法提取海参的DNA,PCR扩增线粒体COⅠ基因并测序,利用Primer premier 5.0软件设计并筛选了仿刺参(Apostichopus japonicus)、北大西洋瓜参(Cucumaria frondosa)、加州红参(Parastichopus californicus)及梅花参(Thelenota ananas)4种海参的特异性探针,设计并进行斑点杂交实验。实验结果显示:4条探针均具有高度的特异性,灵敏度可达100pg,能够实现对仿刺参、北大西洋瓜参、加州红参和梅花参的准确鉴定。本文建立的斑点杂交方法,为海参品种的快速、准确的鉴定提供了可靠的技术方法。  相似文献   

Estimation of the minimum food requirement using the respiration rate of medusa of Aurelia aurita in Sihwa Lake     

Chang-hoon Han  Jinho Chae  Jonghyeok Jin  Wonduk Yoon 《Ocean Science Journal》2012,47(2):155-160

We examined the respiration rate of Aurelia aurita medusae at 20 °C and 28 °C to evaluate minimum metabolic demands of medusae population in Sihwa Lake, Korea during summer. While weight specific respiration rates of medusae were constant and irrespective to the wet weight (8?C220 g), they significantly varied in respect to temperatures (p<0.001, 0.11±0.03 mg C g^?1 of medusa d^?1 at 20°C and 0.28±0.11 mg C g^?1 of medusa d^?1 at 28 °C in average, where Q ₁₀ value was 2.62). The respiration rate of medusae was defined as a function of temperature (T, °C) and body weight (W, g) according to the equation, R=0.13×2.62^(T-20)/10 W ^0.93. Population minimum food requirement (PMFR) was estimated from the respiration rate as 15.06 and 4.86 mg C m^?3 d^?1 in June and July, respectively. During this period, increase in bell diameter and wet weight was not significant (p=1 in the both), suggesting that the estimated PMFR closely represented the actual food consumption in the field. From July to August, medusae grew significantly at 0.052 d-1, thus the amount of food ingested by medusae population in situ was likely to exceed the PMFR (1.27 mg C m^?3 d^?1) during the period. In conclusion, the medusae population of higher density during June and July had limited amount of food, while those of lower in July and August ingested enough food for growth.  相似文献   

Impacts of mariculture practices on the temporal distribution of macrobenthos in Sandu Bay,South China   总被引：3，自引：0，他引：3  

周进 《中国海洋湖沼学报》2012,30(3):388-396

Sandu Bay is located in the East China Sea and is characterized by high-density fish farming and kelp culture. Despite this, little is known about the impacts of these different mariculture practices on the local environment. We investigated the temporal variation in macrobenthos and environmental conditions at three sites in the bay (fish farming site, kelp culture site, and a control site). We collected water and sediment samples during nine cruises between May 2009 and February 2010. The density of macrobenthos peaked at the fish farming site in July (655 ind./m2) whereas density did not fluctuate as widely at the other two sites. Biomass varied significantly at both the control and kelp culture sites, but had only a single peak at the fish farming site in June (21.90 g/m2). The dominant species varied throughout the study period at the control and kelp culture sites, whereas a single terebellid species (Lysilla pacifica) dominated the macrobenthos at the fish farming site. The diversity index H’ increased at the control site beginning in February then decreased after May, whereas H’ was low at the other sites in December. The mean dissolved oxygen level was highest at the control site (6.59 mg/L) and lowest at the fish farming site (5.54 mg/L). DO levels were lowest at all sites in summer (July and August). The sediment acid volatile sulfide content was higher at the fish farming site (1.46 mg/g dry weight) than those at the kelp culture and control sites (1.22 and 0.14 mg/g, respectively). Our results suggest that mariculture practices have a clear impact on the benthic environment/ community in Sandu Bay.  相似文献